OP0024 DECREASED LEVELS OF T FOLLICULAR HELPER (CD4+CXCR5+) CELLS AND CD27+CD38+ AND CD27+CD38- B CELLS IN ANKYLOSING SPONDYLITIS PATIENTS CORRELATE WITH MARKER OF INFLAMMATION

Background: The role of different lymphocyte subsets in ankylosing spondylitis (AS) is still to be elucidated. It has previously been reported contradictory data concerning the levels T Follicular Helper (TFH) cells and differentiated B peripheral blood AS patients. In addition, connection disease related parameters fully revealed. Objectives: purpose this study was investigate level CD4+TFH CD27+CD38+/CD38- patients with from northern Sweden compare age sex-matched controls. We also studied associations between these cell factors. Methods: Peripheral mononuclear (PBMSc) a cohort 50 Region Västerbotten (mean 52±9.1 years, 33 (66 %) men, (100 HLAB27 positive) pair wise matched donor controls 54±8.8 men) were stained combination antibodies allowing for detection CD27, CD38, CD19, CD3, CD4 CXCR5 markers analyzed by flow cytometry. patient examined spinal x-ray radiographic alterations assessed mSASSS. CRP ESR measured physical function activity registered BASMI BASFI respectively ASDAS-CRP BASDAI. Results: When comparing wise, we observed on average 50% reduction TFH (CD3+CD4+CXCR5+) among CD45+ lymphocytes PBMCs (p=0,000008). Furthermore, 20-30% memory/plasma (CD19+CD27+CD38+ CD19+CD27+CD38-) (p=0,002 p=0,007 respectively). For female correlation (R s =-0,551 p=0,022) observed. Moreover, negative correlations two =–0,476 p=0,053 R =–0,522 p=0,032 Conclusion: reduced correlated (CD27+CD38+ CD27+CD38-) cells. inflammation marker negatively as well Our observations indicates humoral immune response AS. Disclosure Interests: None declared